The Walking Dead: Survival Instinct Barksdale area is unlocked as soon as Daryl leaves Lemon Hill accompanied by Merle. Unlike the previous zone, Barksdale is smaller, and it includes only one TWD collectible.
The Walking Dead: Survival Instinct Stuffed Squirrel in Barksdale is not hard to find, as the one in the previous mission, but it can be missed if you dont pay close attention to the environment, and you dont explore a specific building.
The following TWD: Survival Instinct video game guide reveals the exact location of the hidden Squirrel in this area.
Home of Jakes Bar and the notorious Savage Sons Motorcycle Club, Barksdale was a ramshackle stretch of urban decay even prior to the outbreak.
Barksdale Stuffed Squirrel Location
After you arrive in Barksdale your first objective is to find Merles gang in the bar. Exit the backyard and when you reach the main street, stop and look around.
Across the street you should see a large building and a banner on which you can clearly read: Donuts. Next to the said banner you can also see a sign with the following words on it: For Lease.
Before searching for Merles gang, cross the street and enter the building mentioned above; but be careful because the Walkers will wait for you inside. As you enter the front door, in the first chamber you can see a counter. On the right wall, behind the counter is a hole.
Step through the hole and you will reach another chamber with a large bar, on which you can notice several green monitors.
Jump over the bar and look behind it, on the other side, for the Barksdale TWD: Survival Instinct Stuffed Squirrel. Grab the collectable, and then continue to play the mission, to unlock two additional zones: Lafferty and Archer Creek.